- Scientific manager: Béatrice Turcq
- Technical manager: Valérie Prouzet-Mauleon
CRISP’edit
You want to use CRISPR-Cas9 technology to:
- Genetic KO
- Modify a gene sequence
- Perform functional screening (CRISPR libraries)
Presentation
The CRISP'edit platform helps you with the design or carries out your modifications
The CRISP’edit platform helps you with the design or makes your modifications.
Several points will be evaluated for a successful use of CRISPR technology: the vectorization strategy inside the cells, the best technique according to the project (double-stranded or single-stranded cut of DNA, base-editing, prime-editing) , the best screening strategy for modified cells, the different stages of a functional screening project (CRISPR banks).
CRISP'edit services
Services and advice
Design and realization of KO / KI / Punctual Mutations experiments
Design and execution of experiments using sgRNA libraries, screen selection, sgRNA sequencing, bioinformatics analysis of results
Choosing the appropriate CRISPR system
sgRNA design, recombination templates
Vectorization strategy according to the cellular model
Efficacy Screening Strategy
CRISPR-Cas9 technology enables targeted DNA cutting in living cells. This cut will be repaired with the appearance of errors (Indels) or by using a recombination matrix. This system is very powerful when the targeting of Cas9 is effective thanks to suitably designed gRNAs and when the vectorization means (plasmid DNA, ribonucleoprotein, lentivirus) is adapted to the project and the cell type.
CONTACT
- crispedit@u-bordeaux.fr
Scientific officer
Béatrice Turcq
- beatrice.turcq@u-bordeaux.fr
- 05.57.57.10.51
Technical manager
Valérie Prouzet-Mauleon
- valerie.prouzet-mauleon@u-bordeaux.fr
- 05.57.57.45.47
Engineer Assistant
Christelle Saint-Marc
- Christelle.Saint-Marc@u-bordeaux.fr
- 05.57.57.45.47
Engineer Assistant
Noé Calais-Yager
- noe.calais-yager@u-bordeaux.fr
- 05.57.57.45.47
Localisation
CRISP’edit- UAR TBMCore
Bât BBS 4ème étage
Bureau N4029
2 rue Dr Hoffmann Martinot
33000 Bordeaux