- RT-PCR & Single Cell Libraries
- Technical Manager: Xavier Gauthereau
OneCell
- Quantify, qualify nucleic acids
- Perform high-throughput real-time quantitative PCR
- Automate the preparation of quantitative PCR plates
- Prepare “Single cell” libraries for NGS sequencing
OneCell Services
Advice, training and custom service
Advice for the extraction of
nucleic acids
Quantification and qualification of nucleic acids
Quantitative real-time PCR 96 and 384 wells
Automated preparation of PCR plates
Results analysis
ΔΔCq, Standard Curve, Melte Curve
Preparation of “Single cell” libraries intended
to sequencing
Support for NGS sequencing and bioinformatics analyzes
About Onecell
ONECELL provides state-of-the-art equipment and guides users in their studies of gene expression. ONECELL allows the extraction of nucleic acids (DNA and RNA) from tissues (Tissue Lyser Qiagen), their qualification and quantification (QuBit, Bioanalyse Tape Station Agilent), and their large-scale amplification by Real-time high-throughput PCR (Robot EpMotion 5073 Eppendorf, CFX 96 and 384 well BioRad, BioRad deepwell), as well as the generation of libraries for single-cell multiomics analyzes (Chromium Controller 10X Genomics). ONECELL guides the users for the experimental implementation, the use of the devices and the analysis of the PCR results, and ensures the quality control of the nucleic acids and the “Single Cell” libraries generated upstream of the sequencing. ONECELL also provides a custom service ranging from RNA and DNA qualification to PCR and analysis of results.
equipment
- Tissue Lyser Qiagen
- mDrop Denovix
- QuBit
- Bioanalyser Tape Station 2200 Agilent
- Robot EpMotion 5073 Eppendorf
- CFX 96 and 384 well Bio-Rad
- CFX 96 Deep Well Bio-Rad
- Chromium Controller 10X Genomics
Chromium Controller 10X Genomics
Single-cell gene expression
Workflow
Source : 10x Genomics
The Gem Generation
Microfluidics is a technology that manipulates fluids in microchannels. Thus, we can isolate each cell in a micro-droplet of oil containing reagents (polymerase, oligonucleotide, retrotranscriptase, etc.) and a particular bead allowing specific encoding of each transcript called GEM (Gel bead in EMulsion).
Source : 10x Genomics
Source: Dolomite Microfluidics
Source : 10x Genomics
Visium Spatial 10X Genomics
Source : 10x Genomics
example of building libraries
Chromium Single Cell 3' Gene Expression Dual Index Library
Chromium Single Cell 3' Cell Surface Protein Dual Index Library
Contact
Access to the building and the cytometry platform is secured by a digicode. Ask the manager for the codes on your first visit.
Localisation
Real-time quantitative PCR platform
UMS 3427 / US 005 Laboratory
Bat 1B 2nd floor
Carreire Zone Nord
University of Bordeaux
146, rue Léo Saignat
33076 BORDEAUX Cedex